Differential effects of late gestation maternal overnutrition on the regulation of surfactant maturation in fetal and postnatal life

Abstract

With the increasing incidence of obesity worldwide, the proportion of women entering pregnancy overweight or obese has increased dramatically. The fetus of an overnourished mother experiences numerous metabolic changes that may modulate lung development and hence successful transition to air-breathing at birth. We used a sheep model of maternal late gestation overnutrition (LGON; from 115 d gestation, term 147 ± 3 d) to determine the effect of exposure to an increased plane of nutrition in late gestation on lung development in the fetus (at 141 d gestation) and the lamb (30 d after birth). We found a decrease in the numerical density of surfactant protein positive cells, as well as a reduction in mRNA expression of surfactant proteins (SFTP-A, -B and -C), a rate limiting enzyme in surfactant phospholipid synthesis (phosphate cytidylyltransferase 1, choline, alpha; PCYT1A) and glucose transporters (SLC2A1 and SLC2A4) in the fetal lung. In lambs at 30 d after birth, there were no differences between Control and LGON groups in the surfactant components that were downregulated in the LGON fetuses. However, mRNA expression of SFTP-A, PCYT1A, peroxisome proliferator activated receptor-γ (PPARG), fatty acid synthase (FASN) and fatty acid transport protein (FATP1) were increased in LGON lambs compared to controls. These results indicate a reduced capacity for surfactant production in late gestation. While these deficits are normalised by 30 d after birth, the lungs of LGON lambs exhibited altered glucose transport and fatty acid metabolism, which is consistent with an enhanced capacity for surfactant synthesis and restoration of surfactant maturity in these animals.

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