GSE108619 A whole genome-screening strategy to identify DNA2 binding sites and genomic regions that require DNA2 for replication

Contributors : Zhengke Li ; Xiwei Wu ; Binghui Shen
Series Type : Genome binding/occupancy profiling by high throughput sequencing
Organism : Homo sapiens

We report that DNA2 predominantly bound to the centromeric α-satellite regions. The centromeric regions contained 58% of the DNA2-associated DNA, representing a 33.5-fold enrichment over genomic input. To define the under-replicated DNA regions in DNA2-null cells, we conducted whole-genome DNA sequencing of the under-replicated BrdU negative DNA. After normalization to genomic input DNA from the same cells, 12.9% of the peaks from the under-replicated DNA aligned with the centromeric DNA regions, representing an 8.5-fold enrichment. In addition, among the peaks that overlapped between the DNA2 pull-down and under-replicated regions, two thirds fell into the centromeric regions, representing a 48-fold enrichment.

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