Pathogenesis investigation of miR‐199‐5p in oral submucous fibrosis based on bioinformatics analysis

Abstract

Objectives

Fibrosis diseases are one of the leading causes of suffering and death. However, no systematic investigation has been carried out on fibrosis‐related genes.

Materials and Methods

By querying PubMed using keywords "fibrosis" and "gene" or "protein", we identified fibrosis‐related genes in the last decade. Bioinformatics analysis was performed by MAS 3.0 software. Key miRNA was selected to assesse its relationship with oral submucous fibrosis (OSF) and fibroblast functions.

Results

1310 genes related to fibrosis were identified. TGF‐β1, CTGF, MMP9, HSP47 and S1P were found to be associated with mainly fibrotic organs. 244 cellular components terms, 595 molecular function terms, 1816 cellular component terms, 136 KEGG pathway annotations were predicted. MiR‐199‐5p was selected as the key miRNA, which has higher level in OSF. Upregulated miR‐199‐5p was significantly related to OSF duration and OSF histological grade (P = 0.028 and 0.012, respectively). Overexpressive miR‐199‐5p reduced proliferation and induced apoptosis in buccal fibroblasts. Additionally, expression levels of collagen I (COL I) and III (COL III) were promoted by overexpressive miR‐199‐5p in buccal fibroblasts.

Conclusion

These results indicate that fibrosis‐related genes are related to a series of complex mechanisms. The characteristics of miR‐199‐5p may supply important clues for developing therapeutic strategy for OSF.

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