Publication date: Available online 21 December 2018
Source: Dental Materials
Author(s): Berdan Aydin, Ariene A. Leme-Kraus, Cristina M.P. Vidal, Thaiane R. Aguiar, Rasika S. Phansalkar, Joo-Won Nam, James B. McAlpine, Shao-Nong Chen, Guido F. Pauli, Ana K. Bedran-Russo
Abstract
Objectives
The interactivity of proanthocyanidins (PACs) with collagen modulates dentin matrix biomechanics and biostability. Herein, PAC extracts selected based on structural diversity were investigated to determine key PAC features driving sustained effects on dentin matrices over a period of 18 months.
Methods
The chemical profiles of PAC-rich plant sources, Pinus massoniana (PM), Cinnamomum verum (CV) and Hamamelis virginiana (HV) barks, as well as Vitis vinifera (VV) seeds, were obtained by diol HPLC analysis after partitioning of the extracts between methyl acetate and water. Dentin matrices (n = 15) were prepared from human molars to determine the apparent modulus of elasticity over 18 months of aging. Susceptibility of the dentin matrix to degradation by endogenous and exogenous proteases was determined by presence of solubilized collagen in supernatant, and resistance to degradation by bacterial collagenase, respectively. Data were analyzed using ANOVA and Games–Howell post hoc tests (α = 0.05).
Results
After 18 months, dentin matrices modified by PM and CV extracts, containing only non-galloylated PACs, were highly stable mechanically (p < 0.05). Dentin matrices treated with CV exhibited the lowest degradation by bacterial collagenase after 1 h and 18 months of aging (p < 0.05), while dentin matrices treated with PM showed the least mass loss and collagen solubilization by endogenous enzymes over time (p < 0.05).
Significance
Resistance against long-term degradation was observed for all experimental groups; however, the most potent and long-lasting dentin biomodification resulted from non-galloylated PACs.
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