Using FFPE Tissue in Genomic Analyses: Advantages, Disadvantages and the Role of Biospecimen Science
Abstract Purpose of Review Formalin-fixed, paraffin-embedded (FFPE) tissue blocks are considered by some to be suboptimal for Next Generation Sequencing (NGS) because, compared with fresh-frozen (FF) biospecimens, the nucleic acid obtained from them is fragmented and low in quantity, containing formalin-induced modifications and mutations. However, clinical workflows do not typically have the capability to collect FF tissue, so there is...
Abstract Purpose of Review Peripheral blood mononuclear cells (PBMCs) are used in a wide variety of preclinical assays. Preanalytical variations can have a devastating impact on the results. In this review, we list critical preanalytical factors for PBMC-based assays to develop awareness and orientation to the types of sample preparation and storage that one may consider employing. ...
Abstract Purpose of Review In this review, we highlight the importance of using standardized procedures and protocols for circulating miRNA isolation, detection, and quantification, in order to establish their role as non-invasive biomarkers in the clinical setting. Recent Findings Specific miRNAs have already been investigated as promising...
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