GSE100515 Genome-wide strategies identify molecular niches regulated by connective tissue-associated transcription factors [ChIP-seq TFs]

Contributors : Mickael Orgeur ; Stefan T Börno ; Bernd Timmermann ; Jochen Hecht ; Delphine Duprez ; Sigmar Stricker
Series Type : Genome binding/occupancy profiling by high throughput sequencing
Organism : Gallus gallus

To gain insight into the regulatory cascades involved in connective tissue differentiation, we selected five zinc finger transcription factors - OSR1, OSR2, EGR1, KLF2 and KLF4 - based on their expression patterns and/or known involvement in the differentiation of mesenchymal cells into connective tissue subtypes. We combined RNA-seq with ChIP-seq profiling in chick limb cells following overexpression of individual transcription factors. We identified a set of common genes regulated by all five transcription factors, which constitutes a connective tissue core expression set. This common core was enriched in genes associated with axon guidance and myofibroblast signature. In addition, each of the transcription factors regulated a different set of extracellular matrix components and signalling molecules, which define local molecular niches important for connective tissue development and function.

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