Abstract
Background: The recent appreciation of the energy burning capacity of brown adipose tissue turns it to an attractive target as anti-obesity therapy.
Objective: to evaluate the effect of L-carnosine on browning of white adipose tissue in exercised obese rats.
Methods: Sixty adult male Wistar albino rats between 7–8 weeks-old weighing 130–150 g were allocated into six groups;(i) normal control rats fed normal diet; (ii) high fat diet (HFD)-induced obese rats, (iii) normal control rats fed normal diet and injected with L-carnosine (250mg/kg), (iv) HFD-rats injected with L-carnosine (250mg/kg),(v): HFD-rats subjected to exercise training; (vi): HFD- rats subjected to exercise training and L-carnosine together. At the end of the experiment, the body weights and the serum levels of lipid profile, oxidative stress and inflammatory markers as well as circulating myokines are investigated. Gastrocnemius muscles and abdominal adipose tissues were excised for the measurement of gene expression of muscle irisin, adipose tissue uncoupling protein1 (UCP1), p38MAPK and CD137. In addition, histopathological examination for the studied groups was performed.
Results: Both exercise training and carnosine treatment significantly decreased body weight gain, ameliorated the induced dyslipidemia, reduced the thiobarbituric acid reactive species (TBARS) and TNF-?, while increased total antioxidant capacity and IL-10. Furthermore, serum irisin levels and relative. expression of adipose uncoupling protein-1 (UCP-1), adipose CD137 and fibronectin type III domain-containing protein 5(FNDC5), the precursor of irisin gene expression, were associated with the former improvements. The highest improvement was evident in the combined exercise and carnosine group.
Conclusion: These findings suggest that L-carnosine may induce browning of adipose tissue through irisin stimulation, a phenomenon that could be related to its antioxidant, anti-inflammatory and anti-obesity effect. This article is protected by copyright. All rights reserved
from # All Medicine by Alexandros G. Sfakianakis via alkiviadis.1961 on Inoreader http://ift.tt/2BkxDr8
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