GSE102350 Substrate stiffness-dependent gene expression profile for cortical neurons

Contributors : Ting-Ya Chang ; Chen Chen ; Ya-Chu Chang ; Chi-Huan Lu ; Min Lee ; Shao-Tzu Lu ; De-Yao Wang ; Aijun Wang ; Chin-Lin Guo ; Pei-Lin Cheng
Series Type : Expression profiling by array
Organism : Rattus norvegicus

We undertook mRNA microarray and gene ontology analyses to screen out substrate stiffness-dependent genes. Total mRNA were extracted from E17 cortical neurons grown on soft or stiff substrates at 5 or 16 hr time points. We identified 114 differentially-expressed mRNA transcripts in cells grown on 0.1 kPa and 20 kPa gels at the 5 hr time-point. Among them, 66 were upregulated in 0.1 kPa gel cultures and the remainder were downregulated (compared to cells grown on stiffer substrates). The expressions of three endocytic genes (Cltc, Dab2, and Myo6) and four adhesion genes(Vcl, Robo2, Nrcam, and Cad11) were confirmed by QGP and smRNA FISH.

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