GSE99257 Robust detection of chromosomal contacts from small cell numbers using low-input Capture-C

Contributors : A M Oudelaar ; James O Davies ; Damien J Downes ; Douglas R Higgs ; Jim R Hughes
Series Type : Other
Organism : Mus musculus

Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This hampers the investigation of chromatin structure in rare cell populations. We present two new low-input Capture-C protocols that generate high-quality, reproducible interaction profiles from fewer than 20,000 cells, and show that these are not biased by PCR amplification or the degree of formaldehyde fixation.

from # All Medicine by Alexandros G. Sfakianakis via alkiviadis.1961 on Inoreader http://ift.tt/2mOk5hE