Two cases of septic shock with different outcomes caused by non-O1/non-O139 Vibrio cholerae isolates
Journal of International Medical Research, Volume 48, Issue 6, June 2020. In recent decades, increasing numbers of human infections have been linked to non-O1/non-O139 Vibrio cholerae. Septicemia resulting from non-O1/non-O139 V. cholerae infection is rare but has high mortality. The pathogenesis of non-O1/non-O139 V. cholerae septicemia is poorly understood. Here, we report two sporadic cases of septicemia following non-O1/non-O139 V. cholerae infection from an inland area of China. Patient 1 died...
Tue Jun 23, 2020 08:43
Journal of International Medical Research, Volume 48, Issue 6, June 2020. Spontaneous cerebrospinal fluid (sCSF) leak from the skull base has been previously reported, but there are few reports of sCSF leak from the foramen rotundum due to its rare occurrence. This case report describes a 15-year-old male patient that presented with left side watery rhinorrhoea that had been present since he was 4 years of age and a history of repeated bouts of meningitis of unknown cause. A discharge sample from...
Tue Jun 23, 2020 08:40
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveTo investigate adipocytokine expression levels, platelet-to-lymphocyte ratio (PLR) and transforming growth factor (TGF)-β1/Smad signaling activity in diabetic patients with pulmonary infection.MethodsEighty-two type 2 diabetic patients with pulmonary infection were included in the observation group and 75 patients with simple type 2 diabetes were recruited into the control group. The fasting blood glucose (FBG), glycated...
Mon Jun 22, 2020 14:55
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectivesTo investigate the effect of tumor necrosis factor ligand-related molecule 1A (TL1A) on the intestinal mucosal barrier in mice with chronic colitis.MethodsMale TL1A-overexpressing transgenic mice and male C57BL/6 wild-type mice were used to establish a dextran sodium sulfate (DSS)-induced colitis model. The expression of occludin and claudin-1 was observed. Bacterial distribution in the intestinal mucosa and Th9/interleukin...
Mon Jun 22, 2020 14:52
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveThis study was performed to analyze the clinical, radiographic, and pathological features of hand angioleiomyoma causing nerve compression and assess the outcomes of surgical excision.MethodsThis case series included three men and one woman (mean age, 53.3 years; range, 49–56 years). The patients’ chief complaint was numbness of the fingers. The patients’ medical histories were reviewed, and the diagnosis of angioleiomyoma...
Mon Jun 22, 2020 14:49
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveIn post-mastectomy radiotherapy, high-conformal techniques are a valid method for determining the dose distribution around a target. However, the proximity of critical structures is a reason for concern. This study aims to evaluate the feasibility of using calibrated cone-beam computed tomography (CBCT) scans as a valid tool for a timely heart dose evaluation.MethodsA retrospective analysis was conducted on 170 retrospective...
Mon Jun 22, 2020 14:45
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectivesThis study aimed to investigate the factors affecting the quantity of DNA and RNA extractable from human formalin-fixed paraffin-embedded (FFPE) tissues stored for different lengths of time.MethodsWe randomly selected 20 FFPE specimens harvested from hysteromyoma patients with uterine fibroids during 2010, 2015, and 2017 at the Department of Pathology, Jiading District Central Hospital Affiliated Shanghai University...
Mon Jun 22, 2020 14:42
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveSchizophrenia is a severe neurodevelopmental disorder with a complex genetic and environmental etiology. The gene encoding EF-hand domain-containing protein D2 (EFHD2) may be a genetic risk locus for schizophrenia.MethodsWe genotyped four EFHD2 single-nucleotide polymorphisms (281 schizophrenia cases [SCZ], 321 controls) from northern Chinese Han individuals using Sanger sequencing and polymerase chain reaction-restriction...
Mon Jun 22, 2020 13:32
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveHepatocellular carcinoma (HCC) is a common cancer with a high mortality rate; the molecular mechanism involved in HCC remain unclear. We aimed to provide insight into HCC induced with HepG2 cells and identify genes and pathways associated with HCC, as well as potential therapeutic targets.MethodsDataset GSE72581 was downloaded from the Gene Expression Omnibus, including samples from mice injected in liver parenchyma...
Mon Jun 22, 2020 13:29
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveThis study aimed to evaluate the joint monitoring of somatosensory evoked potentials (SEPs) and motor evoked potentials (MEPs) in vertebral canal decompression surgery for acute spinal cord injury.MethodsTwenty-four patients, who were admitted to the hospital for the surgical treatment of spinal cord injury with SEP and MEP monitoring, were assigned to the intraoperative monitoring group (group I). In addition, 24...
Mon Jun 22, 2020 12:02
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveTo explore the glucagon-like peptide-1 analogue liraglutide in the hospital setting in patients with type 2 diabetes mellitus (T2DM) and acute coronary syndrome and to evaluate the safety and efficacy and its impact on hospitalization and short-term glycemic variability (GV).MethodsA 12-week, open-label, prospective, randomized pilot clinical study with parallel groups that compared liraglutide (group 1) with glargine...
Mon Jun 22, 2020 09:55
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveBronchiectasis is a common chronic airway disease. We investigated the economic burden and associated factors of bronchiectasis in China.MethodsIn this multicenter retrospective cohort study, we reviewed medical records of patients admitted to 18 tertiary hospitals during 2010 to 2014 with a bronchiectasis-related diagnosis.ResultsA total 5469 patients with bronchiectasis were admitted, accounting for 3.13% ± 1.80%...
Mon Jun 22, 2020 09:43
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveTo evaluate the association between dyslipidaemia and Alzheimer’s disease (AD) in a cohort of postmenopausal women.MethodsThis retrospective study analysed data from postmenopausal women with early AD (group AD) and a cohort of healthy age- and sex-matched control subjects (group NC) that were considered to be within standard limits according to a neuropsychological assessment between March 2010 and March 2019. The...
Mon Jun 22, 2020 08:42
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectivesCaregiver burden in neurologic Wilson disease (NWD) has received little attention. We investigated predictors of caregiver burden in Chinese NWD patients.MethodsParticipants in this retrospective study were NWD patients admitted to The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine from 1 August to 31 December 2019. Sociodemographic information was recorded for caregivers and NWD patients....
Mon Jun 22, 2020 04:06
Journal of International Medical Research, Volume 48, Issue 6, June 2020. ObjectiveWe aimed to explore the educational outcome and influencing factors of ongoing verbal rehabilitation training together with inclusive education among prelingually deaf children with a cochlear implant.MethodsPrelingually deaf children who underwent cochlear implantation, rehabilitation, and had inclusive education placement were randomly divided into two groups: one group received continuous verbal rehabilitation training...
Mon Jun 22, 2020 04:02
Related Articles Obtaining Primary Osteocytes through Murine Calvarial Fractionation of GFP-Expressing Osteocytes. J Vis Exp. 2020 Jun 02;(160): Authors: Marahleh A, Kitaura H, Ogawa S, Shen WR, Qi J, Ohori F, Noguchi T, Nara Y, Pramusita A, Kinjo R, Mizoguchi I Abstract The osteocyte, once thought to be a passive resident of the bone given the backstage function of sensing mechanical loading, is now brought to the spotlight and has been...
Tue Jun 23, 2020 18:08
Related Articles Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment. J Vis Exp. 2020 Jun 02;(160): Authors: Lemaire Q, Duhamel M, Raffo-Romero A, Salzet M, Lefebvre C Abstract The neuroinflammatory state of the central nervous system (CNS) plays a key role in physiological and pathological conditions. Microglia, the resident immune cells in the brain, and sometimes the infiltrating...
Tue Jun 23, 2020 18:08
Related Articles Involving Individuals with Developmental Language Disorder and their Parents/Carers in Research Priority Setting. J Vis Exp. 2020 Jun 06;(160): Authors: Chadd KE, Kulkarni AA, Longhurst LM Abstract A protocol for involving individuals presenting with developmental language disorder (DLD) (iDLD) and their parents/carers (iDLDPC) in a research priority setting exercise is presented. iDLD have difficulties with communication...
Tue Jun 23, 2020 18:08
Related Articles Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro. J Vis Exp. 2020 Jun 02;(160): Authors: Mao X, Zhao S Abstract The neural differentiation of mouse embryonic stem cells (mESCs) is a potential tool for elucidating the key mechanisms involved in neurogenesis and potentially aid in regenerative medicine. Here, we established an efficient and low cost method for neuronal differentiation from mESCs in vitro,...
Tue Jun 23, 2020 18:08
Related Articles Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection. J Vis Exp. 2020 Jun 08;(160): Authors: Rengucci C, De Maio G, Menghi M, Benzi F, Calistri D Abstract Nowadays, stool DNA can be isolated and analyzed by several methods. The long fragments of DNA in stool can be detected by a qPCR assay, which provides a reliable probability of the presence of pre-neoplastic or neoplastic colorectal lesions....
Tue Jun 23, 2020 18:08
Related Articles Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples. J Vis Exp. 2020 Jun 08;(160): Authors: Levin Y, Talsania K, Tran B, Shetty J, Zhao Y, Mehta M Abstract Gene expression analysis by RNA sequencing (RNA-seq) enables unique insights into clinical samples that can potentially lead to mechanistic understanding of the basis of various diseases as well as resistance and/or susceptibility mechanisms. However,...
Tue Jun 23, 2020 18:08
Related Articles Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy. J Vis Exp. 2020 Jun 04;(160): Authors: Jevtic V, Kindle P, Avilov SV Abstract Mitochondrial nucleoids are compact particles formed by mitochondrial DNA molecules coated with proteins. Mitochondrial DNA encodes tRNAs, rRNAs, and several essential mitochondrial polypeptides. Mitochondrial nucleoids divide and distribute within...
Tue Jun 23, 2020 18:08
Related Articles Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification. J Vis Exp. 2020 Jun 02;(160): Authors: Xu Z, Orkwis JA, Harris GM Abstract Traumatic peripheral nervous system (PNS) injuries currently lack suitable treatments to regain full functional recovery. Schwann cells (SCs), as the major glial cells of the PNS, play a vital role in promoting PNS regeneration by dedifferentiating...
Tue Jun 23, 2020 18:08
Related Articles Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons. J Vis Exp. 2020 Jun 03;(160): Authors: Mao WF, Wu YF Abstract Peripheral epitendinous sutures are believed to enhance core suture strength in tendon repair and decrease the risk of gapping between tendon ends. Here Q suture, an alternative to peripheral sutures, is presented for the use in tendon repair. Its effects on...
Tue Jun 23, 2020 18:08
Related Articles Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay. J Vis Exp. 2020 Jun 07;(160): Authors: Chester EM, Fender E, Wasserman MD Abstract Plants are a source of food for many animals, and they can produce thousands of chemicals. Some of these compounds affect physiological processes in the vertebrates that consume them, such as endocrine function. Phytoestrogens, the most well studied endocrine-active...
Tue Jun 23, 2020 18:08
Related Articles External Cephalic Version: Is it an Effective and Safe Procedure? J Vis Exp. 2020 Jun 06;(160): Authors: Sánchez-Romero J, García-Soria V, Araico-Rodríguez F, Herrera-Giménez J, Blanco-Carnero JE, Nieto-Díaz A, Sánchez-Ferrer ML Abstract External cephalic version (ECV) is an effective procedure for reducing the number of cesarean sections. To date, there is no video publication showing the methodology of this procedure....
Tue Jun 23, 2020 18:08
Related Articles The Effect of Construction and Demolition Waste Plastic Fractions on Wood-Polymer Composite Properties. J Vis Exp. 2020 Jun 07;(160): Authors: Lahtela V, Hyvärinen M, Kärki T Abstract Construction and demolition waste (CDW), including valuable materials such as plastics, have a remarkable influence on the waste sector. In order for plastic materials to be re-utilized, they must be identified and separated according to their...
Tue Jun 23, 2020 18:08
Related Articles The Rigid Tube as an Alternative in Controlling the Problematic Airway. J Vis Exp. 2020 Jun 06;(160): Authors: Marchis IF, Radeanu D, Pop S, Pop A, Cosgarea M Abstract The unexpected problematic airway represents a large proportion of anesthesia-related morbidity and mortality. The retromolar or paraglossal approach is an alternative to the majority of the rigid instruments used for tracheal intubation, which follow the...
Tue Jun 23, 2020 18:08
Related Articles Calibration of Vector Network Analyzer for Measurements in Radio Frequency Propagation Channels. J Vis Exp. 2020 Jun 02;(160): Authors: Hammerschmidt C, Johnk RT, Tran S Abstract In situ measurements of radio frequency (RF) spectrum activity provide insight into the physics of radio frequency wave propagation and validate existing and new spectrum propagation models. Both of these parameters are essential to supporting...
Tue Jun 23, 2020 18:08
Related Articles Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages. J Vis Exp. 2020 Jun 02;(160): Authors: Mangione F, Martin-Blanco E Abstract Within multicellular organisms, mature tissues and organs display high degrees of order in the spatial arrangements of their constituent cells. A remarkable example is given by sensory epithelia, where cells of the same or distinct...
Tue Jun 23, 2020 18:08
Related Articles Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking. J Vis Exp. 2020 Jun 08;(160): Authors: Adhikari S, Banerjee C, Moscatelli J, Puchner EM Abstract Single molecule localization microscopy (SMLM) techniques overcome the optical diffraction limit of conventional fluorescence microscopy and can resolve intracellular structures and the dynamics of biomolecules with ~20 nm...
Tue Jun 23, 2020 18:08
Related Articles Laparoscopic Radical Left Pancreatectomy for Pancreatic Cancer: Surgical Strategy and Technique Video. J Vis Exp. 2020 Jun 06;(160): Authors: Vissers FL, Zwart MJW, Balduzzi A, Korrel M, Lof S, Abu Hilal M, Besselink MG Abstract Radical resection margins, resection of Gerota's (perirenal) fascia, and adequate lymph node dissection are crucial for an adequate oncological resection of left-sided pancreatic cancer. Several...
Tue Jun 23, 2020 18:08
Related Articles Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains. J Vis Exp. 2020 Jun 03;(160): Authors: Poloni TE, Medici V, Carlos AF, Davin A, Ceretti A, Mangieri M, Cassini P, Vaccaro R, Zaccaria D, Abbondanza S, Bordoni M, Fantini V, Fogato E, Cereda C, Ceroni M, Guaita A Abstract In a constantly aging population, the prevalence of neurodegenerative disorders is expected to rise. Understanding...
Tue Jun 23, 2020 18:08
Related Articles Induction and Characterization of Pulmonary Hypertension in Mice using the Hypoxia/SU5416 Model. J Vis Exp. 2020 Jun 03;(160): Authors: Bikou O, Hajjar RJ, Hadri L, Sassi Y Abstract Pulmonary Hypertension (PH) is a pathophysiological condition, defined by a mean pulmonary arterial pressure exceeding 25 mm Hg at rest, as assessed by right heart catheterization. A broad spectrum of diseases can lead to PH, differing in their...
Tue Jun 23, 2020 18:08
Related Articles Measurement of Spatial Stability in Precision Grip. J Vis Exp. 2020 Jun 04;(160): Authors: Teshima R, Noguchi N, Fujii R, Kondo K, Tanaka K, Lee B Abstract The purpose of the protocol is to indirectly evaluate the direction of the finger force during manipulation of a handheld object based on the biomechanical relationships in which deviated force direction causes center of pressure (COP) replacement. To evaluate this,...
Tue Jun 23, 2020 18:08
Related Articles Practical Guide for Identification of Internal Carotid Artery During Endoscopic Nasopharyngectomy. Laryngoscope. 2020 Jun 22;: Authors: Battaglia P, Eesa M, Pietrobon G, Karligkiotis A, Castelnuovo P, Zanoni MT PMID: 32569391 [PubMed - as supplied by publisher]
Tue Jun 23, 2020 18:07
Related Articles Early Feasibility Studies of Augmented Reality Navigation for Lateral Skull Base Surgery. Otol Neurotol. 2020 Jun 18;: Authors: Creighton FX, Unberath M, Song T, Zhao Z, Armand M, Carey J Abstract HYPOTHESIS: An augmented reality (AR) platform using a head mounted display (HMD) can successfully display and register virtual computerized tomography (CT) data to corresponding real world anatomy using fiducial markers. ...
Tue Jun 23, 2020 18:07
Related Articles Current Management of CPAP After Otologic and Neurotologic Surgery. Otol Neurotol. 2020 Jun 19;: Authors: Cass ND, Babu SC Abstract BACKGROUND: Obstructive sleep apnea is a highly prevalent disorder often treated with continuous positive airway pressure (CPAP). CPAP transmits high pressures through the Eustachian tube, and has significant implications for patients undergoing surgery of the middle ear, inner ear, and lateral...
Tue Jun 23, 2020 18:07
Related Articles Assessment of the Optic Foramen Shape and Size in Human Fetuses. J Craniofac Surg. 2020 Jun 18;: Authors: Beger O Abstract OBJECTIVE: This study was aimed to obtain data on the dimensions of the optic foramen in human fetuses for early childhood surgeries. METHODS: Twenty-five formalin-fixed fetuses (16 boys and 9 girls) with average age 21.68 ± 3.12 gestational weeks (range, 16-28 weeks) in the inventory of Anatomy...
Tue Jun 23, 2020 18:07
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