Active commuting to and from school (ACS) has been recognized as a potential tool to improve physical fitness. Thus, this study aims to test the relationships between the average slope in the ACS and physical fitness, as well as to verify the mediator role of fatness in the relationship between average slope and physical fitness. A total of 257 participants, 137 boys and 120 girls, from 22 schools belonged to first and second High School grades participated in this study. Based on self-reported measure...
Tue Jun 09, 2020 03:00
Mycoheterotrophic plants are highly specialized species able to acquire organic carbon from symbiotic fungi, with relaxed dependence on photosynthesis for carbon fixation. The relaxation of the functional constraint of photosynthesis and thereby the relaxed selective pressure on functional photosynthetic genes usually lead to substantial gene loss and a highly degraded plastid genome in heterotrophs. In this study, we sequenced and analyzed the plastome of the eudicot Exacum paucisquama, providing...
Tue Jun 09, 2020 03:00
Background Silicon (Si) is a beneficial element that has been proven to influence plant responses including growth, development and metabolism in a hormetic manner. Methods In the present study, we evaluated the effect of Si on the growth and concentrations of chlorophylls, total amino acids, and total sugars of pepper plants (Capsicum annuum L.) during the early developmental stage in a hydroponic system under conventional (unstressed) conditions. We tested four Si concentrations (applied...
Tue Jun 09, 2020 03:00
Background Subjective Well-Being (SWB) is usually conceptualized in terms of an affective (i.e., judgements of biological emotional reactions and experiences) and a cognitive component (i.e., judgements of life satisfaction in relation to a psychological self-imposed ideal). Recently, researchers have suggested that judgements of harmony in life can replace or at least complement the cognitive component of SWB. Here, however, we go beyond that suggestion and propose that harmony in life should...
Tue Jun 09, 2020 03:00
Since 2014, Stony Coral Tissue Loss Disease (SCTLD) has led to mass mortality of the majority of hard coral species on the Florida Reef Tract. Following the successful treatment of SCTLD lesions on laboratory corals using water dosed with antibiotics, two topical pastes were developed as vehicles to directly apply antibiotic treatments to wild corals. These pastes were tested as placebos and with additions of amoxicillin on active SCTLD lesions on multiple coral species. The effectiveness of the...
Tue Jun 09, 2020 03:00
Over a century of research on between-person differences has resulted in the consensus that human cognitive abilities are hierarchically organized, with a general factor, termed general intelligence or “g,” uppermost. Surprisingly, it is unknown whether this body of evidence is informative about how cognition is structured within individuals. Using data from 101 young adults performing nine cognitive tasks on 100 occasions distributed over six months, we find that the structures of individuals’ cognitive...
Tue Jun 09, 2020 03:00
Background Every human being carries with them a collection of microbes, a collection that is likely both unique to that person, but also dynamic as a result of significant flux with the surrounding environment. The interaction of the human microbiome (i.e., the microbes that are found directly in contact with a person in places such as the gut, mouth, and skin) and the microbiome of accessory objects (e.g., shoes, clothing, phones, jewelry) is of potential interest to both epidemiology...
Tue Jun 09, 2020 03:00
SMRT sequencing of the full-length transcriptome of the white-backed planthopper Sogatella furcifera
The white-backed planthopper Sogatella furcifera is an economically important rice pest distributed throughout Asia. It damages rice crops by sucking phloem sap, resulting in stunted growth and plant virus transmission. We aimed to obtain the full-length transcriptome data of S. furcifera using PacBio single-molecule real-time (SMRT) sequencing. Total RNA extracted from S. furcifera at various developmental stages (egg, larval, and adult stages) was mixed and used to generate a full-length transcriptome...
Tue Jun 09, 2020 03:00
Background Coronavirus disease 19 (COVID-19) is a global health threat with significant medical, economic, social and political implications. The optimal strategies for combating COVID-19 have not been fully determined and vary across countries. Methods By the end of February 2020 in Taiwan, 2,150 patients received diagnostic COVID-19 testing and 39 confirmed cases were detected. This is a relatively lower rate of infection compared to other Asian countries. In this article, we summarize...
Tue Jun 09, 2020 03:00
Insufficient light intensity inhibits the growth of cultivated herbaceous peony and decreases its economic value. Owing to the increased demand for shade-tolerant herbaceous peony, the selection of appropriate parents for hybridization is essential. Paeonia anomala, Paeonia intermedia and Paeonia veitchii can grow under shade conditions in their natural habitats; however, their photosynthetic capacities under shade have not been studied. In this study, we simulated low light intensity (30% sunlight)...
Tue Jun 09, 2020 03:00
Related ArticlesPrimary Nasopharyngeal Tuberculosis: A Case Report Focused on Nasopharyngoscopic Features and CT Findings. Ear Nose Throat J. 2020 Jun 08;:145561320931951 Authors: Min HJ, Kim KS Abstract Primary nasopharyngeal tuberculosis, defined as an isolated tuberculosis infection of the nasopharynx without systemic or pulmonary disease, is rare, even in areas endemic for tuberculosis. It is challenging for ENT specialists to diagnose primary...
Tue Jun 09, 2020 15:59
Related ArticlesMicroendoscopic Surgery of Middle Ear and Petrous Bone: Benefits Analysis. Ear Nose Throat J. 2020 Jun 08;:145561320930017 Authors: Poletti AM, Solimeno LS, Cugini G, Miceli S, Colombo G Abstract OBJECTIVES: Endoscopy has become routinely used in middle ear surgery. The aim of this study is to analyze where this tool may complement the traditional microscopic approach. This is a retrospective study done in single tertiary hospital....
Tue Jun 09, 2020 15:59
Related ArticlesUse of Endoscopic Cartilage Graft Myringoplasty Without Tympanomeatal Flap Elevation to Repair Posterior Marginal Perforations. Ear Nose Throat J. 2020 Jun 08;:145561320931220 Authors: Lou Z Abstract OBJECTIVE: We evaluated the graft success rate and hearing outcomes of endoscopic cartilage graft myringoplasty without tympanomeatal flap elevation used to repair posterior marginal perforations. STUDY DESIGN: A prospective...
Tue Jun 09, 2020 15:59
Related ArticlesLocation of the Sphenoid Sinus Ostium in Relation to Adjacent Anatomical Landmarks. Ear Nose Throat J. 2020 Jun 08;:145561320927907 Authors: Doubi A, Albathi A, Sukyte-Raube D, Castelnuovo P, Alfawwaz F, AlQahtani A Abstract PURPOSE: The variability of sphenoid pneumatization and its relationship with the surrounding structures has been suggested. The aim of this study was to examine the effect of the surrounding bony structures on...
Tue Jun 09, 2020 15:59
Related ArticlesInfectious Mononucleosis and Upper Airway Obstruction: Intracapsular Tonsillectomy and Adenoidectomy With Microdebrider for Prompt Relief. Ear Nose Throat J. 2020 Jun 08;:145561320930046 Authors: Lloyd AM, Reilly BK PMID: 32511006 [PubMed - as supplied by publisher]
Tue Jun 09, 2020 15:59
Related ArticlesLaser Microsurgery Versus Radiotherapy Versus Open Partial Laryngectomy for T2 Laryngeal Carcinoma: A Systematic Review of Oncological Outcomes. Ear Nose Throat J. 2020 Jun 08;:145561320928198 Authors: Campo F, Zocchi J, Ralli M, De Seta D, Russo FY, Angeletti D, Minni A, Greco A, Pellini R, de Vincentiis M Abstract INTRODUCTION: The aim of the current systematic review is to update the pooled survival outcome of patients with T2...
Tue Jun 09, 2020 15:59
Abstract Purpose of Review To review sleep complaints reported in patients with autoimmune encephalitis, explore the relationship between sleep disturbances and subtypes of autoimmune encephalitis, and leverage knowledge concerning antibody-antigen specificity to inform the receptors, structures, and disseminated neural networks that contribute to sleep function in health and disease. ...
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Related ArticlesElucidation of the Molecular Mechanisms Underlying Sorafenib-Induced Hepatotoxicity. Oxid Med Cell Longev. 2020;2020:7453406 Authors: AlAsmari AF, Ali N, AlAsmari F, AlAnazi WA, Alqahtani F, Alharbi M, Alotaibi FM, Aldossari AA, AlSwayyed M, Alanazi MM, Alshamrani AA Abstract Sorafenib is a small, orally-active multikinase inhibitor that is most frequently used for the management of renal cell carcinoma, hepatocellular carcinoma,...
Tue Jun 09, 2020 16:27
Related ArticlesLooking under the hood of "the Cadillac of cancers:" radioactive iodine-related craniofacial side effects among patients with thyroid cancer. J Cancer Surviv. 2020 Jun 06;: Authors: Diamond-Rossi SA, Jonklaas J, Jensen RE, Kuo C, Stearns S, Esposito G, Davidson BJ, Luta G, Bloom G, Graves KD Abstract PURPOSE: Despite having a generally favorable prognosis, differentiated thyroid cancer is known to have a significant, long-term impact...
Tue Jun 09, 2020 16:27
Abstract Persistent homology is constrained to purely topological persistence while multiscale graphs account only for geometric information. This work introduces persistent spectral theory to create a unified lowdimensional multiscale paradigm for revealing topological persistence and extracting geometric shapes from high‐dimensional datasets. For a point‐cloud dataset, a filtration procedure is used to generate a sequence of chain complexes and associated families of simplicial complexes and chains,...
Mon Jun 08, 2020 18:16
Related ArticlesMuscle BDNF: a potential therapeutic target for kennedy's disease. J Physiol. 2020 Jun 07;: Authors: Whitley KC, Brown AD, Ryan CR, Cleverdon REG PMID: 32506451 [PubMed - as supplied by publisher]
Tue Jun 09, 2020 16:22
Related ArticlesBiologic validation of EPR image oxygen thresholds in tissue. J Physiol. 2020 Jun 07;: Authors: Gertsenshteyn I, Giurcanu M, Vaupel P, Halpern H Abstract Measuring molecular oxygen levels in vivo has been the cornerstone of understanding the effects of hypoxia in normal tissues and malignant tumors. Here we discuss the advances in a variety of partial pressure of oxygen (pO2 ) measurements and imaging techniques and relevant oxygen thresholds....
Tue Jun 09, 2020 16:22
Related ArticlesAnodal and cathodal tDCS modulate neural activity and selectively affect γ-aminobutyric acid and glutamate syntheses in the visual cortex of cats. J Physiol. 2020 Jun 07;: Authors: Zhao X, Ding J, Pan H, Zhang S, Pan D, Yu H, Ye Z, Hua T Abstract KEY POINTS: This study showed that anodal and cathodal transcranial direct current stimulation (tDCS) can respectively increase and decrease the amplitude of visually evoked field potentials...
Tue Jun 09, 2020 16:22
Related ArticlesCochlear implantation in children under 12 months: Prevalence and implications of 'hidden' disabilities. Cochlear Implants Int. 2020 Jun 07;:1-6 Authors: Friedmann DR, Tona KM, Roland JT, Spitzer ER, Waltzman SB Abstract Introduction: While cochlear implants (CI) prior to 12 months of age have become common, the prevalence and impact of issues that either arise or were not evident prior to implantation is unknown. Methods: Retrospective...
Tue Jun 09, 2020 16:20
Related ArticlesDark as night: Spelunking for spinal solitary fibrous tumors/hemangiopericytomas in the differential of T2 hypointensity. J Clin Neurosci. 2020 Jun 04;: Authors: Carlstrom LP, Graffeo CS, Perry A, Atkinson JD, Lanzino G, Meyer FB, Morris JM Abstract Spinal solitary fibrous tumor/hemangiopericytoma (SFT/HPC) is a rare mesenchymal malignancy. Radiographically, SFT/HPCs have a mutable appearance, with irregular borders, heterogeneous contrast...
Tue Jun 09, 2020 16:13
Related ArticlesGender differences in use of prolonged non-operative therapies prior to index ACDF surgery. J Clin Neurosci. 2020 Jun 02;: Authors: Davison MA, Lilly DT, Moreno J, Bagley C, Adogwa O Abstract Prior to anterior cervical discectomy and fusion (ACDF) surgery, patients suffering from cervical stenosis traditionally trial non-operative treatments for pain management. There is a paucity of data evaluating gender disparities in the prolonged...
Tue Jun 09, 2020 16:13
Related ArticlesDoes COVID-19 cause axonal GBS? J Clin Neurosci. 2020 May 23;: Authors: Umapathi T PMID: 32505432 [PubMed - as supplied by publisher]
Tue Jun 09, 2020 16:13
Related ArticlesNeuroimmune correlates of the nervous system involvement of COVID-19: A commentary. J Clin Neurosci. 2020 May 27;: Authors: Yesilkaya UH, Balcioglu YH PMID: 32505431 [PubMed - as supplied by publisher]
Tue Jun 09, 2020 16:13
Related ArticlesA New Bony Anatomical Landmark for Lateral Skull Base Surgery. J Craniofac Surg. 2020 Jun;31(4):1157-1160 Authors: Liu Z, Yi Z Abstract BACKGROUND AND OBJECTIVE: The aim of the study was to propose a new bony landmark, point O, which is the junction point of the temporosphenoid suture and the infratemporal ridge; this landmark may improve the identification and preservation of neurovascular structures when approaches are used to access...
Tue Jun 09, 2020 16:09
Related ArticlesChanging paradigms in sinus and skull base surgery as the COVID-19 pandemic evolves: Preliminary experience from a single Italian tertiary care center. Head Neck. 2020 Jun 08;: Authors: Karligkiotis A, Arosio AD, Battaglia P, Sileo G, Czaczkes C, Volpi L, Turri-Zanoni M, Castelnuovo P Abstract BACKGROUND: Italy was the first European country suffering from COVID-19. Health care resources were redirected to manage the pandemic. We present...
Tue Jun 09, 2020 16:09
Related ArticlesComprehensive review of the mastoid foramen. Neurosurg Rev. 2020 Jun 07;: Authors: Wang C, Lockwood J, Iwanaga J, Dumont AS, Bui CJ, Tubbs RS Abstract Anatomical variations of the mastoid foramen have been observed to vary in a number of qualities including size, number, and location. These variants have the potential to become problematic during surgical approaches to the posterior cranial fossa and mastoid part of the temporal bone,...
Tue Jun 09, 2020 16:09
Related ArticlesEndoscopy-assisted transoral resection of a parapharyngeal space schwannoma without mandibular dissection. Auris Nasus Larynx. 2020 Jun 05;: Authors: Yamamoto K, Kurose M, Yadomura R, Yajima R, Okuni T, Takano K Abstract Of the schwannomas that arise from the parapharyngeal space, those in the high cervical region are particularly invasive, requiring mandibular dissection. Because these tumors are benign, however, excessive surgical invasion...
Tue Jun 09, 2020 16:09
Related ArticlesCaution in the use of TissuePatchDS-P™ in drainless benign superficial parotidectomy, a pilot study: our experience in three patients. Clin Otolaryngol. 2020 Jun 07;: Authors: Bird JH, Mirza A, King EV Abstract Benign parotid surgery is a common Head & Neck procedure often requiring up to two nights as an inpatient, however fibrin glue products are allowing for drainless parotid surgery. TissuePatchDS-PTM is a synthetic adhesive patch...
Tue Jun 09, 2020 16:09
Related ArticlesSurgical management of middle cranial fossa bone defects: meningoencephalic herniation and cerebrospinal fluid leaks. Am J Otolaryngol. 2020 May 28;41(4):102560 Authors: Hernandez-Montero E, Caballero E, García-Ibanez L Abstract Along the medical practice of an Otologist he/she will face middle cranial fossa (MCF) bone defects. The purpose of this study is to contribute to the understanding of this possible life threatening condition,...
Tue Jun 09, 2020 16:09
Related ArticlesAn emergency medicine-focused review of malignant otitis externa. Am J Emerg Med. 2020 Apr 29;: Authors: Long DA, Koyfman A, Long B Abstract INTRODUCTION: Malignant otitis externa (MOE) is a progressive infection of the external auditory canal (EAC). This disease is rare but has severe morbidity and mortality. OBJECTIVE: This narrative review provides an overview of malignant otitis externa for emergency clinicians. ...
Tue Jun 09, 2020 16:09
Related ArticlesA minimally invasive endoscopic transnasal retropterygoid approach to the upper parapharyngeal space: anatomic studies and surgical implications. Int Forum Allergy Rhinol. 2019 11;9(11):1263-1272 Authors: Liu J, Sun X, Liu Q, Gu Y, Li H, Zheng C, Wang D, Fernandez-Miranda JC, Snyderman CH, Yu H Abstract BACKGROUND: Surgery remains the mainstay of treatment for lesions in the parapharyngeal space. However, gaining access to the parapharyngeal...
Tue Jun 09, 2020 16:09
Related ArticlesReconstructing Denisovan Anatomy Using DNA Methylation Maps. Cell. 2019 Sep 19;179(1):180-192.e10 Authors: Gokhman D, Mishol N, de Manuel M, de Juan D, Shuqrun J, Meshorer E, Marques-Bonet T, Rak Y, Carmel L Abstract Denisovans are an extinct group of humans whose morphology remains unknown. Here, we present a method for reconstructing skeletal morphology using DNA methylation patterns. Our method is based on linking unidirectional methylation...
Tue Jun 09, 2020 16:09
Related ArticlesParapharyngeal space paraganglioma: distinguishing vagal paragangliomas from carotid body tumours using standard MRI. Clin Radiol. 2019 Sep;74(9):734.e1-734.e6 Authors: Wang X, Chen Y, Chen X, Xian J Abstract AIM: To evaluate whether standard magnetic resonance imaging (MRI) could distinguish vagal paragangliomas (VPs) from carotid body tumours (CBTs) in the parapharyngeal space. METHODS AND MATERIALS: Thirteen VPs in 13 patients...
Tue Jun 09, 2020 16:09
Related ArticlesValidated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples. J Vis Exp. 2020 May 19;(159): Authors: Reckers A, Wen A, Aguilar D, Bacchetti P, Gandhi M, Metcalfe J, Gerona R Abstract Drug resistant-tuberculosis (DR-TB) is a growing public health threat, and assessment of therapeutic drug levels may have important clinical benefits. Plasma drug levels are the current gold standard assessment, but...
Tue Jun 09, 2020 16:46
Related ArticlesMeasuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System. J Vis Exp. 2020 May 22;(159): Authors: Restrepo S, Schwartz SL, Kennedy MJ, Aoto J Abstract Protein interactions at cellular interfaces dictate a multitude of biological outcomes ranging from tissue development and cancer progression to synapse formation and maintenance. Many of these fundamental interactions occur in trans and are typically...
Tue Jun 09, 2020 16:46
Related ArticlesAssessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry. J Vis Exp. 2020 May 19;(159): Authors: Funk KE, Lotz SK Abstract Increasing evidence supports the hypothesis that neuro-immune interactions impact nervous system function in both homeostatic and pathologic conditions. A well-studied function of major histocompatibility complex class I (MHCI) is the presentation...
Tue Jun 09, 2020 16:46
Related ArticlesIsolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells. J Vis Exp. 2020 May 19;(159): Authors: DiSano KD, Linzey MR, Welsh NC, Meier JS, Pachner AR, Gilli F Abstract The central nervous system (CNS) is comprised of the brain and spinal cord and is enveloped by the meninges, membranous layers serving as a barrier between the periphery and the CNS. The CNS is an immunologically specialized...
Tue Jun 09, 2020 16:46
Related ArticlesDeciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation. J Vis Exp. 2020 May 20;(159): Authors: Tamirat MZ, Kurppa KJ, Elenius K, Johnson MS Abstract Numerous somatic mutations occurring in the epidermal growth factor receptor (EGFR) family (ErbB) of receptor tyrosine kinases (RTK) have been reported from cancer patients, although relatively few have been tested and shown to cause...
Tue Jun 09, 2020 16:46
Related ArticlesControlling Electrospun Polymer Morphology for Tissue Engineering Demonstrated Using hepG2 Cell Line. J Vis Exp. 2020 May 25;(159): Authors: Bate TSR, Forbes SJ, Callanan A Abstract Electrospinning affords researchers the opportunity to fabricate reproducible micro to nanoscale polymer fibers. The 3D fibrous architecture of electrospun polymers is regarded as a structural imitation of the extracellular matrix (ECM). Hence, electrospun...
Tue Jun 09, 2020 16:46
Related ArticlesMeasuring Sub-23 Nanometer Real Driving Particle Number Emissions Using the Portable DownToTen Sampling System. J Vis Exp. 2020 May 22;(159): Authors: Bainschab M, Landl L, Andersson J, Mamakos A, Hausberger S, Bergmann A Abstract The current particle size threshold of the European Particle Number (PN) emission standards is 23 nm. This threshold could change because future combustion engine vehicle technology may emit large amounts...
Tue Jun 09, 2020 16:46
Related ArticlesRapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection. J Vis Exp. 2020 May 24;(159): Authors: Vipat S, Brennan G, Park C, Haller SL, Rothenburg S Abstract Vaccinia virus (VACV) was instrumental in eradicating variola virus (VARV), the causative agent of smallpox, from nature. Since its first use as a vaccine, VACV has been developed as a vector for therapeutic vaccines and as an oncolytic virus....
Tue Jun 09, 2020 16:46
Related ArticlesAssessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies. J Vis Exp. 2020 May 22;(159): Authors: Petriello A, Becerra JA, Lay J, Husaini R, Windler SL, Duramad O, Liu SD Abstract One mechanism of action for clinical efficacy by therapeutic antibodies is the promotion of immune-related functions, such as cytokine secretion and cytotoxicity, driven by FcγRIIIa (CD16) expressed...
Tue Jun 09, 2020 16:46
Related ArticlesLoop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation. J Vis Exp. 2020 May 20;(159): Authors: Domesle KJ, Young SR, Yang Q, Ge B Abstract Loop-mediated isothermal amplification (LAMP) has emerged as a powerful nucleic acid amplification test for the rapid detection of numerous bacterial, fungal, parasitic, and viral agents. Salmonella is a bacterial pathogen...
Tue Jun 09, 2020 16:46
Related ArticlesConducting Concurrent Electroencephalography and Functional Near-Infrared Spectroscopy Recordings with a Flanker Task. J Vis Exp. 2020 May 24;(159): Authors: Xu SY, Cheong LI, Zhuang Y, Couto TAP, Yuan Z Abstract Concurrent EEG and fNIRS recordings offer an excellent opportunity to gain a full understanding of the neural mechanism of cognitive processing by inspecting the relationship between the neural and hemodynamic signals. EEG...
Tue Jun 09, 2020 16:46
Related ArticlesTransvenous Embolization of Carotid Cavernous Fistula through Inferior Petrosal Sinus with Detachable Coils and Ethylene Vinyl Alcohol Copolymer. J Vis Exp. 2020 May 21;(159): Authors: Gu J, Yan M, Fan W, Liu W, Wang M, Wan S Abstract Carotid cavernous fistula (CCF) is a rare disease caused by abnormal communications between the internal carotid artery (direct fistula) or meningeal branches of the external carotid artery (indirect...
Tue Jun 09, 2020 16:46
Related ArticlesTreatment of Facial Deformities using 3D Planning and Printing of Patient-Specific Implants. J Vis Exp. 2020 May 23;(159): Authors: Shilo D, Capucha T, Goldstein D, Bereznyak Y, Emodi O, Rachmiel A Abstract Technological advancements in surgical planning and patient-specific implants are constantly evolving. One can either adopt the technology to achieve better results, even in the less experienced hand, or continue without it. As...
Tue Jun 09, 2020 16:46
Related ArticlesOpsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens. J Vis Exp. 2020 May 19;(159): Authors: Chua J, Chabot DJ, Putmon-Taylor T, Friedlander AM Abstract The opsono-adherence assay is a functional assay that enumerates the attachment of bacterial pathogens to professional phagocytes. Because adherence is requisite to phagocytosis and killing, the...
Tue Jun 09, 2020 16:46
Related ArticlesIsolation and Expansion of Neurospheres from Postnatal (P1-3) Mouse Neurogenic Niches. J Vis Exp. 2020 May 23;(159): Authors: Soares R, Ribeiro FF, Lourenço DM, Rodrigues RS, Moreira JB, Sebastião AM, Morais VA, Xapelli S Abstract The neurosphere assay is an extremely useful in vitro technique for studying the inherent properties of neural stem/progenitor cells (NSPCs) including proliferation, self-renewal and multipotency. In the...
Tue Jun 09, 2020 16:46
Related ArticlesPurification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro. J Vis Exp. 2020 May 20;(159): Authors: He Q, Yu F, Li Y, Sun J, Ding F Abstract The principal cells in the peripheral nervous system are the Schwann cells (SCs) and the fibroblasts. Both these cells distinctly express the sensory and motor phenotypes involved in different patterns of neurotrophic factor gene expression and other biological processes,...
Tue Jun 09, 2020 16:46
Related ArticlesAn Electrochemiluminescence-Based Assay for MeCP2 Protein Variants. J Vis Exp. 2020 May 22;(159): Authors: Steinkellner H, Beribisky AV, Mausberg P, Christodoulou J, Scheiber-Mojdehkar B, Huber A, Sarne V, Laccone F Abstract The ECLIA is a versatile method which is able to quantify endogenous and recombinant protein amounts in a 96-well format. To demonstrate ECLIA efficiency, this assay was used to analyze intrinsic levels of MeCP2...
Tue Jun 09, 2020 16:46
Related ArticlesLive Cell Imaging of Microtubule Cytoskeleton and Micromechanical Manipulation of the Arabidopsis Shoot Apical Meristem. J Vis Exp. 2020 May 23;(159): Authors: Wang Y, Sampathkumar A Abstract Understanding cell and tissue level regulation of growth and morphogenesis has been at the forefront of biological research for many decades. Advances in molecular and imaging technologies allowed us to gain insights into how biochemical signals...
Tue Jun 09, 2020 16:46
Related ArticlesPreference Assessment of White-backed Planthopper Feeding on Rice. J Vis Exp. 2020 May 23;(159): Authors: Zhang L, Wu C, Xiao Y Abstract Exploiting insect-resistant rice germplasm resources and related genes is the primary need for breeding insect-resistant varieties, but the accuracy of the identification of insect-resistant phenotypes of rice is a major difficulty. It is urgent to develop a new method or improve existing methods...
Tue Jun 09, 2020 16:46
Related ArticlesHigh Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing. J Vis Exp. 2020 May 21;(159): Authors: Zhang JQ, Chang KC, Liu L, Gartner ZJ, Abate AR Abstract The powerful tools available to edit yeast genomes have made this microbe a valuable platform for engineering. While it is now possible to construct libraries of millions of genetically distinct strains, screening for a desired phenotype remains a significant obstacle....
Tue Jun 09, 2020 16:46
Related ArticlesAssessing Early Stage Open-Angle Glaucoma in Patients by Isolated-Check Visual Evoked Potential. J Vis Exp. 2020 May 25;(159): Authors: Fan X, Wu L, Ding A Abstract Recently, the isolated-check visual evoked potential (icVEP) technique was designed and has been reported to detect glaucomatous damage earlier and faster. It creates low spatial frequency/high temporal frequency bright stimuli and records cortical activity initiated primarily...
Tue Jun 09, 2020 16:46
Related ArticlesProtein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans. J Vis Exp. 2020 May 23;(159): Authors: Li L, Zinovyeva AY Abstract Co-immunoprecipitation methods are frequently used to study protein-protein interactions. Confirmation of hypothesized protein-protein interactions or identification of new ones can provide invaluable information about the function of a protein of interest. Some of the traditional methods...
Tue Jun 09, 2020 16:46
Related ArticlesMeasurements of Physiological Stress Responses in C. Elegans. J Vis Exp. 2020 May 21;(159): Authors: Bar-Ziv R, Frakes AE, Higuchi-Sanabria R, Bolas T, Frankino PA, Gildea HK, Metcalf MG, Dillin A Abstract Organisms are often exposed to fluctuating environments and changes in intracellular homeostasis, which can have detrimental effects on their proteome and physiology. Thus, organisms have evolved targeted and specific stress responses...
Tue Jun 09, 2020 16:46
Related ArticlesAn Image Guided Transapical Mitral Valve Leaflet Puncture Model of Controlled Volume Overload from Mitral Regurgitation in the Rat. J Vis Exp. 2020 May 19;(159): Authors: Corporan D, Kono T, Onohara D, Padala M Abstract Mitral regurgitation (MR) is a widely prevalent heart valve lesion, which causes cardiac remodeling and leads to congestive heart failure. Though the risks of uncorrected MR and its poor prognosis are known, the longitudinal...
Tue Jun 09, 2020 16:46
Related ArticlesApplication of Atomic Force Microscopy to Detect Early Osteoarthritis. J Vis Exp. 2020 May 24;(159): Authors: Danalache M, Tiwari A, Sigwart V, Hofmann UK Abstract Biomechanical properties of cells and tissues not only regulate their shape and function but are also crucial for maintaining their vitality. Changes in elasticity can propagate or trigger the onset of major diseases like cancer or osteoarthritis (OA). Atomic force microscopy...
Tue Jun 09, 2020 16:46
Related ArticlesConfocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells. J Vis Exp. 2020 May 20;(159): Authors: Volpe BA, Fotino TH, Steiner AB Abstract Hair cells are mechanosensory cells that mediate the sense of hearing. These cells do not regenerate after damage in humans, but they are naturally replenished in non-mammalian vertebrates such as zebrafish. The zebrafish lateral line system is a useful model...
Tue Jun 09, 2020 16:46
Related ArticlesThe Effect of Anodization Parameters on the Aluminum Oxide Dielectric Layer of Thin-Film Transistors. J Vis Exp. 2020 May 24;(159): Authors: Gomes TC, Kumar D, Alves N, Kettle J, Fugikawa-Santos L Abstract Aluminum-oxide (Al2O3) is a low cost, easily processable and high dielectric constant insulating material that is particularly appropriate for use as the dielectric layer of thin-film transistors (TFTs). Growth of aluminum-oxide...
Tue Jun 09, 2020 16:46
Related ArticlesPreparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue. J Vis Exp. 2020 May 19;(159): Authors: Karabasheva D, Smyth JT Abstract Experimental analysis of cells dividing in living, intact tissues and organs is essential to our understanding of how cell division integrates with development, tissue homeostasis, and disease processes. Drosophila spermatocytes undergoing meiosis are ideal...
Tue Jun 09, 2020 16:46
Glioblastoma multiforme (GBM) has a poor prognosis despite a multi modal treatment that includes normofractionated radiotherapy. So, various hypofractionated alternatives to normofractionated RT have been test...
Mon Jun 08, 2020 03:00
Abstract Background Periodontal infection induces inflammation, which may increase the risk of tumor-promoting effects. The aim of this study was to assess the association between periodontitis and all-cause mortality, and all-cancer and specific cancers’ mortality in a health examination cohort of the elderly in the communities. Methods ...
Tue Jun 09, 2020 03:00
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