Extracellular DNA (ecDNA) released during cell death is proinflammatory and contributes to inflammation. Measurement of ecDNA at the site of injury can determine the efficacy of therapeutic treatment in the target organ. This protocol describes the use of a machine learning tool to automate measurement of ecDNA in kidney tissue.
Thu Jun 18, 2020 22:00
Two fabrication techniques, lift-off and wet etching, are described in producing interdigital electrode transducers upon a piezoelectric substrate, lithium niobate, widely used to generate surface acoustic waves now finding broad utility in micro to nanoscale fluidics. The as-produced electrodes are shown to efficiently induce megahertz order Rayleigh surface acoustic waves.
Thu Jun 18, 2020 21:00
Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions.
Tue Jun 16, 2020 23:00
This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.
Tue Jun 16, 2020 22:00
Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.
Tue Jun 16, 2020 22:00
This protocol describes the procedure for sectioning and culturing human cardiac slices for preclinical drug testing and details the use of optical mapping for recording transmembrane voltage and intracellular calcium signals simultaneously from these slices.
Tue Jun 16, 2020 21:00
We present a methodology to establish the pollination requirements of apricot (Prunus armeniaca L.) cultivars combining the determination of self-(in)compatibility by fluorescence microscopy with the identification of the S-genotype by PCR analysis.
Tue Jun 16, 2020 20:00
The objective is to fractionate and isolate bioactive small molecules, peptides from a complex plant extract, and proteins from pathogenic microbes by employing liquid-phase isoelectric focusing (IEF) method. Further, the separated molecules were identified and their bioactivity confirmed.
Sun Jun 14, 2020 22:00
The footpad inoculation model is a valuable tool for characterizing viral-induced neuroinflammatory responses in vivo. In particular, it provides a clear assessment of viral kinetics and associated immunopathological processes initiated in the peripheral nervous system.
Sun Jun 14, 2020 22:00
Double in utero electroporation allows targeting cell populations that are spatially and temporally separated. This technique is useful to visualize interactions between those cell populations using fluorescent proteins in normal conditions but also after functional experiments to perturb genes of interest.
Sun Jun 14, 2020 21:00
In the present protocol, we explain how to easily process and culture tonsillar mononuclear cells from healthy humans undergoing partial surgical tonsillectomy to study innate immune responses upon activation, mimicking viral infection in mucosal tissues.
Sun Jun 14, 2020 20:00
This publication describes a protocol for the isolation of nuclei from mature adipocytes, purification by fluorescence-activated sorting, and single-cell level transcriptomics.
Fri Jun 12, 2020 22:00
This article presents an efficient method to perform myocardial ischemia and subsequent chronic reperfusion in rats using a minimally invasive approach. In addition, left ventricular hemodynamic function of rats is assessed by echocardiography and isolated working heart methods.
Thu Jun 11, 2020 22:00
This protocol describes an in vivo assay of phagocytosis used to assess and quantify the ability of young and aged Drosophila melanogaster hemocytes to phagocytose bacteria.
Thu Jun 11, 2020 21:00
We describe protocols for assessing the degree of transduction by cell-penetrating peptides utilizing ex vivo imaging systems followed by paraffin embedding, sectioning, and confocal fluorescent microscopy using cardiac targeting peptide as an example. In our protocol, a single animal can be used to acquire both types of imaging assessment of the same organs, thereby cutting the number of animals needed for studies by half.
Thu Jun 11, 2020 21:00
This protocol presents a simple and coherent way to transiently upregulate a gene of interest using modRNA after myocardial infarction in mice.
Thu Jun 11, 2020 20:00
This method describes the steps to improve the quality and quantity of sequence data that can be obtained from formalin-fixed paraffin-embedded (FFPE) RNA samples. We describe the methodology to more accurately assess the quality of FFPE-RNA samples, prepare sequencing libraries, and analyze the data from FFPE-RNA samples.
Mon Jun 08, 2020 23:00
We present an optimized tandem mass tag (TMT) labeling protocol that includes detailed information for each of the following steps: protein extraction, quantification, precipitation, digestion, labeling, submission to a proteomics facility, and data analyses.
Mon Jun 08, 2020 22:00
Conventional BODIPY conjugates can be used for live-cell single-molecule localization microscopy (SMLM) through exploitation of their transiently forming, red-shifted ground state dimers. We present an optimized SMLM protocol to track and resolve subcellular neutral lipids and fatty acids in living mammalian and yeast cells at the nanoscopic length scale.
Mon Jun 08, 2020 21:00
The presented diagnostic FL-DNA kit is a time-saving and user-friendly method to determine the reliable probability of the presence of colorectal cancer lesions.
Mon Jun 08, 2020 20:00
First of all, we would like to thank our colleagues for their kind words regarding our article “Density of the mandibular ramus (cancellous:cortical bone volume ratio) as a predictor of the lingual fracture pattern in bilateral sagittal split osteotomy”.1 In this article, we investigated the association between lingual fracture patterns after bilateral sagittal split osteotomy (BSSO) and the cortical and cancellous bone volume in the mandibular ramus. We found that mandibular angles with a relatively...
Mon Jul 06, 2020 03:00
Asymmetric mandibular prognathism is a clinically common skeletal dentomaxillofacial deformity. Unilateral sagittal split ramus osteotomy (USSRO) is an effective alternative procedure to bilateral sagittal split ramus osteotomy (BSSRO) for some patients. However, the biomechanical effect of temporomandibular joint (TMJ) of USSRO has not been fully studied. This study aims to evaluate the stress distribution changes in the TMJ of asymmetric mandibular prognathism treated with BSSRO/USSRO, to validate...
Mon Jul 06, 2020 03:00
We have read with great interest the article entitled “Density of the mandibular ramus (cancellous:cortical bone volume ratio) as a predictor of the lingual fracture pattern in bilateral sagittal split osteotomy” by van der Hee et al1 in a recent issue of the journal. We appreciate that they have provided us with worthy clinical results and would like to share some of our opinions concerning the timing of third molar removal in relation to bilateral sagittal split ramus osteotomy (BSSO).
Mon Jul 06, 2020 03:00
Traditional model surgery with facebow transfer is not very accurate. We aimed to demonstrate that the Orthopilot™ Navigation System improves the accuracy of maxillary repositioning during Le Fort I osteotomy. Thirty patients underwent Le Fort I osteotomy alone or associated to sagittal split osteotomy. The maxilla positioning was done in two phases. First, the maxilla was positioned with the traditional occlusal splint, the position (“without Orthopilot™”) was recorded by the Orthopilot™. In the...
Mon Jul 06, 2020 03:00
This was a prospective clinical study and the purpose was to assess the quality of life (QoL) as the primary endpoint for patients given prospective treatment for cancer of the oral cavity that required reconstruction using various reconstruction methods in Azerbaijan. All patients who were operated on during the year 2017 to 2018 were entered into the study. Quality of life was assessed six months postoperatively using the European Organization for Research and Cancer Treatment Core Cancer Quality...
Mon Jul 06, 2020 03:00
While there have been previous studies examining the relation between the rs17820943 and rs6072081 polymorphisms in the v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) gene and rates of nonsyndromic cleft lip with or without cleft palate (NSCL/P), at present the results of these studies have been inconsistent. This meta-analysis therefore aimed to conduct a more robust assessment of the association between the MAFB rs17820943 and rs6072081 polymorphisms and NSCL/P risk. The Embase,...
Mon Jul 06, 2020 03:00
The bilateral sagittal split osteotomy is considered the standard surgery to correct facial asymmetries. More recently, unilateral sagittal split osteotomy (USSO) was used to treat such malocclusions.
Mon Jul 06, 2020 03:00
Tissue Engineering Part A, Ahead of Print.
Mon Jul 06, 2020 10:00
Emerging evidence suggests that COVID-19 patients are at a higher risk of stroke and promptly diagnosing and treating such patients is a priority in hospitals across the world. Moreover, identifying which COVID-19 patients are at increased risk of developing a stroke is also important, and may help with preemptive treatment and monitoring. The Lucid Robotic System, developed by LA-based Neural Analytics, is a transcranial doppler system that allows clinicians to identify clots...
Mon Jul 06, 2020 23:45
People that have to use sign language to communicate with others can feel like foreigners in their own land. Very few random strangers can read sign language, but a new technology out of University of California, Los Angeles may make all of us be able to understand someone speaking using signs. The UCLA team developed gloves that use stretchable sensors, that can detect when individual fingers are bent and the hand motions that are produced, to interpret each sign as it is displayed...
Mon Jul 06, 2020 22:48
Abbott won FDA approval for its Gallant implantable cardioverter defibrillator and cardiac resynchronization therapy defibrillator devices. Cleared in Europe earlier this year, the implants feature Bluetooth connectivity, allowing data to stream to a physician for closer observation and monitoring. The devices connect to the myMerlinPulse smartphone app, available for Android and iOS smartphones and tablets, and can share details about adverse events, how the device is performing,...
Mon Jul 06, 2020 18:55
Analyst, 2020, Accepted ManuscriptDOI: 10.1039/D0AN01017K, PaperPieter Dennis van Dalen Luna, Lukasz Majchrzak, Kamilla Malek, Joanna Kuncewicz, Pawel Miskowiec MiskowiecIn the pre-Hispanic Central Andes, the mummified bodies of ancestors stood as the basis for the social and cosmic order. However, the mummification techniques in that region are still poorly...The content of this RSS Feed (c) The Royal Society of Chemistry
Mon Jul 06, 2020 02:00
Analyst, 2020, Accepted ManuscriptDOI: 10.1039/D0AN00604A, PaperZiya Isiksacan, Murat Serhatlioglu, Caglar ElbukenFlow behavior of blood is determined mainly by red blood cell (RBC) deformation and aggregation as well as blood viscoelasticity. These intricately interdependent parameters should be monitored by healthcare providers...The content of this RSS Feed (c) The Royal Society of Chemistry
Mon Jul 06, 2020 02:00
Analyst, 2020, Accepted ManuscriptDOI: 10.1039/D0AN00884B, PaperBenjamin Viada, Lidia Mabel Yudi, Damien W.M. ArriganPer- and polyfluoroalkyl substances (PFAS) are a category of persistent environmental contaminants that have been linked to health issues in humans. In this work, we investigate the detection of perfluorooctanesulfonate...The content of this RSS Feed (c) The Royal Society of Chemistry
Mon Jul 06, 2020 02:00
Key Points BCG vaccination induced a broad range of Th subtypes, including Th1 and Tfh. Vaccine design with BCG epitopes used BCG Th cells to drive Ab responses. Prior BCG exposure can be exploited in a general approach to improve vaccine design.
Mon Jul 06, 2020 23:00
Key Points Synergic effects of LPS and histamine induce jagged junction morphology. Junction morphology drives TEM separately from adhesion molecules or WPBs. S1P and Rap1 signaling pathways reverse jagged junction morphology.
Mon Jul 06, 2020 23:00
The inflammatory response to severe acute respiratory syndrome–related coronavirus 2 infection has a direct impact on the clinical outcomes of coronavirus disease 2019 patients. Of the many innate immune pathways that are engaged by severe acute respiratory syndrome–related coronavirus 2, we highlight the importance of the inflammasome pathway. We discuss available pharmaceutical agents that target a critical component of inflammasome activation, signaling leading to cellular pyroptosis, and the...
Mon Jul 06, 2020 23:00
Key Points IL-6 regulates eosinophilia in a murine model of allergic asthma. Allergic inflammation confers IL-6–dependent protection against S. pneumoniae. IL-6 regulates lung barrier integrity during allergic asthma.
Mon Jul 06, 2020 23:00
Aging impairs immunity to promote diseases, especially respiratory viral infections. The current COVID-19 pandemic, resulting from SARS-CoV-2, induces acute pneumonia, a phenotype that is alarmingly increased with aging. In this article, we review findings of how aging alters immunity to respiratory viral infections to identify age-impacted pathways common to several viral pathogens, permitting us to speculate about potential mechanisms of age-enhanced mortality to COVID-19. Aging generally leads...
Mon Jul 06, 2020 23:00
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